Tyler Golato

Research Scientist

Tyler Golato

Research Scientist

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Hi, I'm Tyler Golato!

Research Scientist

About Me

Biochemistry and Molecular Biology

My background is in biochemistry and molecular biology. As an undergraduate, I worked with Dr. Ron Hutchison and Dr. Daniel Hernandez investigating pathogenesis in limulus egg populations using Next-Generation Sequencing (NGS). Additionally, I worked in the laboratory of Dr. Robert Fine at Columbia University investigating and overcoming mechanisms of chemotherapy resistance in pancreatic cancer. We also worked to develop novel therapeutic regimens for the treatment of neuroendocrine tumors (NETs). My primary interests are DNA damage & repair, aging, cancer biology, epigenetics, and immunology. I am currently an Intramural Research Training Award Fellow (IRTA) at the National Institutes of Health (NIH) – National Institute on Aging (NIA)/Laboratory of Molecular Gerontology (LMG) studying the role of base excision repair in relation to the aging process and age-related pathologies. Additionally, we are working to elucidate the molecular basis of Cockayne Syndrome (CS).

Profile

Full name

Tyler Michael Golato

BORN

19 July 1992 – Philadelphia, U.S.A

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Education

B.S. - Biochemistry and Molecular Biology

Stockton University - Graduation May 2015

Bachelors of Science in Biochemistry and Molecular Biology with a premedical concentration. Research activities include Next-generation Sequencing, pathogenesis in limulus egg populations, and chemotherapy resistance. 

Experimental Therapeutics Internship

Columbia University College of Physicians and Surgeons (2 Years) - May 2013 - May 2015

Under the direction of Dr. Robert L. Fine, M.D., Director of Experimental Therapeutics at the Herbert Irving Comprehensive Cancer Center, New York Presbyterian Hospital- Columbia University Medical Center. 

NIH IRTA Fellow

National Institutes of Health - 2015-2017

National Institute on Aging – Laboratory of Molecular Gerontology. Under the direction of Dr. David M Wilson III – Repair of Endogenous DNA Damage.

Research Appointments

Student Researcher – Stockton University

Multiple Projects - 2011 - 2015

1.Next-generation sequencing and metagenomics analysis were utilized to investigate pathogenicity in Limulus egg populations. 2. Fellowship funded research as an extension of an internship at Columbia University Medical Center. Research was performed exploring the supra-additive properties of various chemotherapy drugs in pancreatic cancer cell lines. 

Summer Program

Cape Regional Medical Center - March 2013 - July 2013

A clinical internship with multi-week rotations in general surgery, orthopedic surgery, radiation oncology, anesthesiology, and primary care. 

Cape Conservation

The Wetlands Institute & NJ Fish and Wildlife - April 2014 - June 2014

Fieldwork and surveying research as an extension of lab work involving next-generation sequencing for the identification of novel pathogenic phenomena in Limulus egg populations.

Clinical Internship

The Pancreas Center - Summer 2013

Columbia University Medical Center – Department of Hematology-Oncology. 20hrs/week in The Pancreas Center at Columbia University Medical Center. A clinical internship with direct access to patients with pancreatic adenocarcinomas and neuroendocrine tumors being treated on clinical study protocols.

Research Internship

Experimental Therapeutics – Research Lab of Dr. Robert Fine - Summer 2014

New York Presbyterian Hospital – Columbia University Medical Center. Extensive laboratory work elucidating mechanisms of chemotherapy resistance in pancreatic adenocarcinoma, neuroendocrine cancers, glioblastoma multiforme, and non-small cell lung cancer cell lines.

Post-baccalaureate IRTA Fellowship

National Institutes of Health - June 2015 - June 2017

Intramural Research Training Award Fellow – 2 year intensive research fellowship in preparation for MD/PhD training. National Institute on Aging – Laboratory of Molecular Gerontology – Repair of Endogenous DNA Damage

Research Projects

NIH/NIA

Lab of Molecular Gerontology - 2015-2017

Variability in base excision repair (BER) capacity may inform aging and age-related pathologies. National Institute on Aging – Laboratory of Molecular Gerontology, Repair of Endogenous DNA Damage Section
Creation of a high-throughput assay to measure variability in BER capacity in the context of aging.

NIH/NIA

Lab of Molecular Gerontology - 2015-2017

Elucidating the molecular mechanisms of Cockayne Syndrome (CS). National Institute on Aging – Laboratory of Molecular Gerontology, Repair of Endogenous DNA Damage Section
Research to understand the molecular basis of Cockayne Syndrome in an effort to identify molecular targets for novel therapeutics and to better understand aging and age-related pathology.

Columbia College of Physicians and Surgeons

Experimental Therapeutics - April 2014 - Present

Chemosensitization of pancreatic adenocarcinoma using 5-FU to activate hENT1 – under the direction of Dr. Robert L. Fine, M.D., Director of Experimental Therapeutics at the Herbert Irving Comprehensive Cancer Center, New York Presbyterian Hospital-Columbia University Medical Center
An exploration of the role of 5-FU in upregulating the human equilibrative nucleoside transporter (hENT1) in multiple pancreatic adenocarcinoma cell lines. hENT1 is responsible for transporting Gemcitabine and other nucleoside analogs into the cell. It serves as a prognostic marker in pancreatic cancer, and its expression is correlated with a stronger response to Gemcitabine. Contribution: cell-culture, real-time PCR, flow cytometry and Western Blots. This ongoing work has supported the development of the GTX chemotherapy regimen for pancreatic cancer (PC). The GTX regimen consists of three drugs — Gemzar, Taxatere, and Xeloda — and is currently in Phase II clinical trials. (Manuscript in preparation)

Columbia College of Physicians and Surgeons

Experimental Therapeutics - April 2014 - Present

Overcoming mechanisms of drug resistance in neuroendocrine tumors (PNETs, carcinoid, medullary thyroid, pituitary, NSCLC) and glioblastoma multiforme – under the direction of Dr. Robert L. Fine, M.D., Director of Experimental Therapeutics at the Herbert Irving Comprehensive Cancer Center, New York Presbyterian Hospital-Columbia University Medical Center
Understanding and manipulating mechanisms of drug resistance in neuroendocrine tumors and glioblastomas. 5-FU was used to deplete O6-alkylguanine DNA alkyltransferases and potentiate the effects of methylator/alkylator drugs in multiple neuroendocrine and glioblastoma multiforme cell lines. Contribution: cell-culture, real-time PCR, flow cytometry and Western Blots. This ongoing work has supported the development of the CapTem (capecitabine and temozolomide) regimen for the treatment of neuroendocrine tumors. CapTem has showed extraordinary promise in early clinical trials.

Columbia College of Physicians and Surgeons

Experimental Therapeutics - April 2014 - August 2014

The restoration of full apoptotic function by a novel C-terminal p53 palindromic tetrapeptide – under the direction of Dr. Robert L. Fine, M.D., Director of Experimental Therapeutics at the Herbert Irving Comprehensive Cancer Center, New York Presbyterian Hospital- Columbia University Medical Center
A novel p53 palindromic tetrapeptide that restores apoptotic function to mutant p53 cancer cells was developed and tested in vitro and in vivo, exogenously and endogenously. Contribution: cell culture, flow cytometry, apoptosis assays and manuscript preparation. (Manuscript in preparation)

Columbia College of Physicians and Surgeons

Experimental Therapeutics - April 2014 - August 2014

Retrospective chart review study on benefit of above-standard dose Octreotide LAR in patients with neuroendocrine tumors – Herbert Irving Comprehensive Cancer Center, New York Presbyterian Hospital-Columbia University Medical Center.The identification and genome sequence of a novel Betaproteobacteria associated with pathogenicity in Limulus eggs – under the direction of Dr. Ron Hutchison and Dr. Daniel Hernandez
Recently, a phenomenon has been observed in which Limulus eggs turn red, coinciding with a cessation of development. Next-generation sequencing was utilized to analyze microbial diversity in both healthy and afflicted horseshoe crab eggs. 16S/18S universal primers allowed for both prokaryotic and eukaryotic analysis. Sequences representing the metagenome of the limulus egg were obtained from healthy and afflicted eggs. The microbiome of each egg population was compared using QIIME (Quantitative Insights Into Microbial Ecology). Additionally, a novel betaproteobacteria believed to be responsible for pathogenesis was isolated and its genome was sequenced. (Manuscript in preparation)

Stockton University

Chemotherapeutic Synergy - 2014-2015

Funded by the Robert Fine Fellowship for Cancer Research – First project involving human cancer cells performed at Stockton University – an extension of Experimental Therapeutics internship at Columbia. Elucidating the role of 5-FU in upregulating the human equilibrative nucleoside transporter (hENT1) in multiple pancreatic adenocarcinoma cell lines.

Research Skills

Biochemistry and Molecular Biology

DNA & RNA isolation & purification
Sanger sequencing
Next-generation sequencing (Ion Torrent)
PCR
qRT-PCR
Flow cytometry (FACS analysis)
Enzyme assays
Protein purification
Electrophoresis
HPLC
GC-MS
Microarrays
Microscopy (Fluorescent and Confocal)
Western blotting
Gram staining
Tissue & cell culture
Metagenomics
Assay Design
Bioinformatics – QUIIME, Spades, Galaxy

Bibliography

Presentations

Stockton University - 2011-2015

1. The Role of CCR5 Antagonists in Slowing the Progression of HIV in Sub-Saharan African Populations. Tyler Golato, Chintan Patel, Amad Akhtar. Stockton College, 2011.
2. The Identification and Genome Sequence of A Novel Betaproteobacteria Associated With Pathogenicity in Limulus Eggs. Tyler Golato, Mark Koester, Chintan Patel, Ron Hutchison and Daniel Hernandez. NAMS Symposium, Stockton College, 2014.
3. Marine Metagenomics: The Limulus Egg Microbiome and Community Changes Associated With The Reg Egg Phenomenon. Tyler Golato, Ron Hutchison and Daniel Hernandez. NAMS Symposium, Stockton University, 2015.
4. 5-Fluorouracil Chemosensitizes Pancreatic Cancer Cells to Gemcitabine by Upregulating the Human Equilibrative Nucleoside Transporter 1 (hENT-1) In Vitro. Biochemistry and Molecular Biology Symposium, Stockton University, 2015.

Professional Memberships

Student Memberships

American Society of Biochemistry and Molecular Biology (ASBMB)
American Society of Clinical Oncology (ASCO)
American Association for the Advancement of Science (AAAS)
Explorer’s Club

Relevant Coursework

Stockton University


Cells and Molecules & Lab
Biodiversity and Evolution & Lab
Genetics & Lab
General Chemistry I & Lab
General Chemistry II & Lab
Organic Chemistry I & Lab
Organic Chemistry II & Lab
Physics I & Lab
Physics II & Lab
Calculus
Molecular Genetics
Biochemistry
Advanced Biochemistry
Physical Chemistry
Immunology
Biochemistry Lab Methods
Psychology
The Biology of Aging
Man, Atom, Universe

Honors & Awards

Stockton University

Robert Fine Fellowship
REU Undergraduate Award
Golden Key Honor Society
Deans List (Every Semester at Stockton)
International Scholar Laureate
Medical Oncology Internship – Columbia University
National Institutes of Health Post-baccalaureate IRTA Fellowship

Volunteer Experience

Humble Volunteers (Helping Us Maintain Better Living Environments)

President and Founder
Nonprofit organization that assists in the mobilization of volunteers for projects that interest them. Focus is science education and conservation. Also provides community clean-ups and free tutoring services. www.humblevolunteers.org

Food Pantry

Green Creek United Methodist Church
Food and clothing distribution to disadvantaged communities each 3rd Wednesday of the month.

Cape Regional Medical Center

Volunteer
Basic patient care duties on a volunteer basis.

Other Employment

Cape Regional Medical Center

- 2011-2013

ER Technician & CNA
Basic patient care duties. Over 2000 hours direct patient contact.

Wildwood Sign and Design

- 2010-Present

Freelance Design Work; Editor for Wildwood & Cape May Properties, Wildwood Magazine
Worked as a designer, both graphic and web. Chief editor of three publications; Wildwood Magazine, Wildwood Properties, and Cape May Properties

Google Business View

- 2014-Present

Instant 360
Photography and sales as an independent contractor for Google Maps, Business View Division

Contact

SEND ME A MESSAGE

ADDRESS

9500 Seaview Avenue, Wildwood Crest, NJ 08260

PHONE

609-972-1573

MAIL

Use the form on this page.

Thank you!

"There are two possible outcomes: if the result confirms the hypothesis, then you've made a measurement. If the result is contrary to the hypothesis, then you've made a discovery."

Enrico Fermi

Background Illustration: The Machinery of Life. By: David S. Goodsell